The depth of the young Redbacks squad was tested when SA stalwarts Travis Head, Alex Carey, Kane Richardson, and Adam Zampa were unavailable due to their inclusion in the Gillette T20I Tri-Series.
Debutant Harry Nielsen and Nick Winter earned their caps as a result, and they rose to the occasion.
'Keeper-batsman Nielsen, batting at no.7, anchored the first innings when the Redbacks were in early trouble, scoring a century in his debut innings after his father Tim Nielsen presented him his cap.
Canberra product and left-arm swing bowler Nick Winter also shone, tearing through Victoria's top-order to claim the tenth best figures of a Redbacks debut bowler with 5-85.
Despite the best efforts of both players in their second innings, Test squad member and Victorian Captain Peter Handscomb found form with an unbeaten century.
Handscomb, who made a seven-ball duck in the first innings, started steadily before bringing up the run-rate in scoring 114 not off 128 balls.
The Vics' captain combined with veteran former skipper Cameron White for a pivotal 138-run stand for the fourth wicket.
White backed up his first-innings' 149 by scoring 50, before being dismissed by part-time spinner Jake Lehmann.
South Australia's bowlers struggled for their usual line and length as they sought wickets, with Joe Mennie (2-40) the pick of the frontline quartet.
Day four began with South Australia 5-208, and boasting a lead of 216.
The Redbacks were not able to add a considerable amount to their overnight score, leaving Victoria with a gettable target off 77 overs.
They next face the New South Wales Blues at the SCG from Friday.